Life Technologies Corporation. It is used in quantitative PCR because the fluorescence can be measured at the end of each amplification cycle to determine, relatively or absolutely, how much DNA has been amplified. Применяется для прокраски гелей (как агарозных, так и полиакриламидных), содержащих двухцепочечную ДНК, а также для realtime-PCR. SYBR Green I binds to DNA.
Dragan AI(1), Pavlovic R, McGivney JB, Casas-Finet JR, Bishop ES, Strouse RJ, Schenerman MA, Geddes CD.
Author information: (1)Institute of Fluorescence, . Since the mix is provided as an easy-to-use real master reagent, reaction setup only . The combination of a hot start and a unique qPCR buffer system ensures highly specific and sensitive real-time quantification of gDNA and cDNA . The dye binds into the minor groove of dsDNA, and does not bind to ssDNA. Благодаря этому его можно использовать в качестве универсального реагента для флуоресцентной детекции амплификации в ПЦР реального времени. Для этого не требуется использовать . Order a Sample or Buy Today!
Summary Quantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the threshold cycle (Ct) during PCR when the level of fluorescence gives signal over the background and is in the linear portion of . Several different chemistries are commonly used for real-time PCR detection. For all chemistries, the amount of emitted fluorescence is proportional to the amount of product (copies of the specific gene) being made in . Use this product when primer design . However, there are several differences between the dyes. Specification, Download specification (PDF). Bacterial DNA, not detectable (min.
PCR cycles). Concentration, 10X concentrated solution. Real-time PCR probes vs. One such application is real-time PCR, also known as qPCR. FastStart Essential DNA Green Master.
For life science research only. Not for use in diagnostic procedures. PCR SybrMaster lowROX PCR-373.
It is used as intercalating dye for the general detection of double-stranded DNA (dsDNA). An additional application . Previous Previous Next.