Its name is often abbreviated to Taq Pol or simply Taq. It is frequently used in the polymerase chain reaction (PCR), a method for . It has a DNA polymerase activity and a exonuclease activity. In addition, Taq DNA Pol.
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It is suitable for applications requiring high temperature synthesis of DNA. Schultz HJ(1), Gochi AM(1), Chia HE(1), Ogonowsky AL(1), Chiang S(1), Filipovic N(1), Weiden AG(1), Hadley EE(1), Gabriel SE(1), Leconte AM(1). It can be used for routine PCR applications including . A versatile thermostable polymerase that can be used for a variety of PCR applications. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time.
Moreover, we found that 30– activity of Taq Pol I was lost at . Thermus aquaticus strain YT-(1).
DNA Polymerase , Taq – Worthington Enzyme Manual. The enzyme is purified from E. Taq DNA polymerase synthesizes DNA from single- stranded . This makes it very suitable for standard applications. Strong red color of the enzyme allows user to check the polymerase addition. For further processing only.
The molecular weight of the recombinant protein is 94kD. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0. You did not add any gift products to the cart. Source, Isolated from E. The robust enzyme is well suited for sensitive experiments using random primers or bacterial templates. This unmodified enzyme replicates DNA at 74ºC and exhibits a half-life of minutes at 95ºC. It works excellent for not only single PCR but also for multiplex PCR.
IBI utilizes a proprietary purification process that in contaminant- and nuclease-free Taq. Highly purified thermostable DNA polymerase for PCR. Provided with Green and Colorless Reaction Buffers.
Green Buffer allows direct gel analysis after amplification.