It is included with Platinum Taq, Taq , and the SuperScript First-Strand Synthesis System for RT-PCR. Find product specific information including CAS, MSDS, protocols and references. An optimal buffer system is essential to perform successful PCR.
Ampliqon has developed different Tris-based buffer solutions to meet different requirements in PCR applications. Why is this necessary for PCR ?
After performing a series of short experiments using the PCR Optimization Kit, choose the combination of stand-alone Buffers A-H that will yield optimal PCR performance specific for your assay. PCR Buffers – Ready-to-use PCR buffers for everyday research. Unit of measure is mL. Application: Use this buffer together with Taq DNA Polymerase. M MgCI(final concentration).
Isopropanol precipitation of DNA and treatment of DNA pellets with ethanol is usually effective in removing traces of contaminants from the DNA sample. PCR buffer is necessary to create optimal conditions for activity of Taq DNA polymerase.
PCR BUFFER 10X(CUSTOM) Find MSDS or SDS, a COA, data sheets and more information. Buffers often contain Tris-Hcl, KCl, and sometimes MgCl2. Water, enzyme buffers and other reagents needed to perform PCR.
Learn more about each option on the individual product pages. PCR buffer optimization with uniform temperature regimen to facilitate automation. Blanchard MM(1), Taillon-Miller P, Nowotny P, Nowotny V. Author information: (1)Washington University School of Medicine, Center for Genetics in Medicine, St. Enough buffer is supplied for 000. Standard PCR buffer , 10x.
NTP Mixture, (25μmol of each dA, dC, dG, dT) Concentration: 100mM, 1ml. PCR reactions of µl each . Molecular Biology Grade, PCR use. HPLC Purity: Store at -degree.
NTP SET, Concentration: 100mM, 4x1ml set of dNTP consisting separate tubes (4x1ml) each with 100mM . Restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.
To order chemicals, medical devices, . Unsure about this product? Product Description: 10X Real-Time PCR buffer 5ml. Individual Container Size: 5ml. Number of Containers: 1. Refrigeration Requirements: Freezer.
Shipping Conditions: ICE. Buffers are solutions designed to maintain the best pH balance (acid or base) for your reaction, in this case PCR. Tris is short for trishydroxymethylaminomethane .